Green Synthesis of Clitoria ternatea L. Silver Nanoparticles into Cotton Fabric for Antibacterial Activity

Authors

S. D. Hasarangi, Department of Cosmetic Sciences, Faculty of Health Sciences, CINEC Campus, Millennium Drive, IT Park, Malabe, Sri Lanka
J. A. L. P. Jayasinghe, Department of Cosmetic Sciences, Faculty of Health Sciences, CINEC Campus, Millennium Drive, IT Park, Malabe, Sri Lanka
S. M. J. C. Abhayasekara, Department of Cosmetic Sciences, Faculty of Health Sciences, CINEC Campus, Millennium Drive, IT Park, Malabe, Sri Lanka
M. S. D. Fernando, Department of Cosmetic Sciences, Faculty of Health Sciences, CINEC Campus, Millennium Drive, IT Park, Malabe, Sri LankaFollow
L. M. Hettihewa, Faculty of Health Sciences, CINEC Campus, Millennium Drive, IT Park, Malabe, Sri Lanka

Document Type

Article

Abstract

Clitoria ternatae L. is a medicinal plant rich in bioactive compounds and green synthesis of silver nanoparticles (AgNPs) using plant extracts offers a sustainable approach with reduced toxicity and minimum environmental impact. In this study, AgNPs were synthesized using aqueous extract of C. ternatae flowers and coated onto cotton fabric to evaluate its antibacterial activity. Dried flower powder (0.02 g) was extracted with distilled water and silver nanoparticles were synthesized using silver nitrate solution (3 mM) under at 50 °C. The nanoparticles were characterized using UV-Vis spectroscopy, Dynamic Light Scattering (DLS), Fourier Transform Infrared Spectroscopy (FTIR). Incorporation on cotton fabric was carried by dip coating method (F₁) & dip coating method followed by sonication (F₂). Antibacterial efficacy of treated fabric was evaluated using the disc diffusion method against Escherichia coli and Staphylococcus aureus. Formation of AgNPs was confirmed by a characteristic surface plasmon resonance peak at 430 nm. The average nanoparticles size was 119.8 nm in size and FTIR analysis indicated the involvement of phenolic and anthocyanin related groups in nanoparticle stabilization. All the treated cotton samples exhibited antibacterial activity and higher inhibition zone observed for F₂ treated fabrics. The mean inhibition zones for F₂ method were 1.5 cm ± 0.05 and 1.4 cm ± 0.05 against Escherichia coli and Staphylococcus aureus respectively. These findings demonstrate the potential of C. ternatae mediated AgNPs for developing antibacterial cotton textiles using environmental applications.

Recommended Citation

Hasarangi, S. D.; Jayasinghe, J. A. L. P.; Abhayasekara, S. M. J. C.; Fernando, M. S. D.; and Hettihewa, L. M. (2026) "Green Synthesis of Clitoria ternatea L. Silver Nanoparticles into Cotton Fabric for Antibacterial Activity," Journal of Innovation in Science, Engineering and Technology: Vol. 7: Iss. 1, Article 6.
Available at: https://jiset.cinec.edu/journal/vol7/iss1/6

Pages

46

Last Page

55